Data Supplement from AR-Regulated TWEAK-FN14 Pathway Promotes Prostate Cancer Bone Metastasis

Supplemental Figure 4. (A) AR activity signature genes expressed as a summed Z score in metastatic samples separated on the basis of Fn14 expression above the median (Fn14 high) or below the median (Fn14 low) using the Grasso dataset. * p<0.05. (B) qChIP analysis was performed in DU145 cells expressing doxycycline induced AR. AR binding to the predicted AREs in the Fn14 promoter was determined either with or without prior DHT (5nM) treatment for 4 hours. The binding activity to each site is given as a percentage of total input then normalized to individual immunoglobulin G (IgG). (C) FACS analysis of human Fn14 promoter reporter in DU145 cells expressing Tet-on TA and TREG3-AR with or without prior doxycycline and DHT treatment.