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Video S1 from ΔNp63-Regulated Epithelial-to-Mesenchymal Transition State Heterogeneity Confers a Leader–Follower Relationship That Drives Collective Invasion

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posted on 2023-03-31, 03:46 authored by Jill M. Westcott, Sharon Camacho, Apsra Nasir, Molly E. Huysman, Raneen Rahhal, Tuyen T. Dang, Anna T. Riegel, Rolf A. Brekken, Gray W. Pearson

Live imaging of clustered spheroids containing SUM159T-dox:Î"Np63 cells {plus minus} doxycycline (H2B:GFP, green, nuclei) showing cell motility.

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NIH

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ARTICLE ABSTRACT

Defining how interactions between tumor subpopulations contribute to invasion is essential for understanding how tumors metastasize. Here, we find that the heterogeneous expression of the transcription factor ΔNp63 confers distinct proliferative and invasive epithelial-to-mesenchymal transition (EMT) states in subpopulations that establish a leader–follower relationship to collectively invade. A ΔNp63-high EMT program coupled the ability to proliferate with an IL1α- and miR-205–dependent suppression of cellular protrusions that are required to initiate collective invasion. An alternative ΔNp63-low EMT program conferred cells with the ability to initiate and lead collective invasion. However, this ΔNp63-low EMT state triggered a collateral loss of fitness. Importantly, rare growth-suppressed ΔNp63-low EMT cells influenced tumor progression by leading the invasion of proliferative ΔNp63-high EMT cells in heterogeneous primary tumors. Thus, heterogeneous activation of distinct EMT programs promotes a mode of collective invasion that overcomes cell intrinsic phenotypic deficiencies to induce the dissemination of proliferative tumor cells. These findings reveal how an interaction between cells in different EMT states confers properties that are not induced by either EMT program alone.