American Association for Cancer Research
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00085472can141042-sup-129649_1_video_2498597_n6jl81.mp4 (1.75 MB)

Supplementary Video #7 from Live-Cell Imaging of Invasion and Intravasation in an Artificial Microvessel Platform

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posted on 2023-03-30, 22:43 authored by Andrew D. Wong, Peter C. Searson

An HMVEC vessel under shear stress (12-15 dyne cm-2) extending an angiogenic sprout towards a cluster of MDA-MB-231 breast cancer cells.

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ARTICLE ABSTRACT

Methods to visualize metastasis exist, but additional tools to better define the biologic and physical processes underlying invasion and intravasation are still needed. One difficulty in studying metastasis stems from the complexity of the interface between the tumor microenvironment and the vascular system. Here, we report the development of an investigational platform that positions tumor cells next to an artificial vessel embedded in an extracellular matrix. On this platform, we used live-cell fluorescence microscopy to analyze the complex interplay between metastatic cancer cells and a functional artificial microvessel that was lined with endothelial cells. The platform recapitulated known interactions, and its use demonstrated the capabilities for a systematic study of novel physical and biologic parameters involved in invasion and intravasation. In summary, our work offers an important new tool to advance knowledge about metastasis and candidate antimetastatic therapies. Cancer Res; 74(17); 4937–45. ©2014 AACR.

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