American Association for Cancer Research
00085472can131641-sup-movie2.mpg (3.1 MB)

Supplementary Movie 2 from VEGF-Mediated Angiogenesis Links EMT-Induced Cancer Stemness to Tumor Initiation

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posted on 2023-03-30, 22:30 authored by Anna Fantozzi, Dorothea C. Gruber, Laura Pisarsky, Chantal Heck, Akiko Kunita, Mahmut Yilmaz, Nathalie Meyer-Schaller, Karen Cornille, Ulrike Hopfer, Mohamed Bentires-Alj, Gerhard Christofori

MPG file - 3174K, Three-dimensional confocal reconstitution.



An epithelial–mesenchymal transition (EMT) underlies malignant tumor progression and metastatic spread by enabling cancer cells to depart from the primary tumor, invade surrounding tissue, and disseminate to distant organs. EMT also enriches for cancer stem cells (CSC) and increases the capacity of cancer cells to initiate and propagate tumors upon transplantation into immune-deficient mice, a major hallmark of CSCs. However, the molecular mechanisms promoting the tumorigenicity of cancer cells undergoing an EMT and of CSCs have remained widely elusive. We here report that EMT confers efficient tumorigenicity to murine breast cancer cells by the upregulated expression of the proangiogenic factor VEGF-A and by increased tumor angiogenesis. On the basis of these data, we propose a novel interpretation of the features of CSCs with EMT-induced, VEGF-A–mediated angiogenesis as the connecting mechanism between cancer cell stemness and tumor initiation. Cancer Res; 74(5); 1566–75. ©2014 AACR.