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Supplementary Movie 2 from The Blebbishield Emergency Program Overrides Chromosomal Instability and Phagocytosis Checkpoints in Cancer Stem Cells
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posted on 2023-03-31, 01:08 authored by Goodwin G. Jinesh, Ashish M. KamatTime-lapse movie showing the exit phase of blebbishield emergency program. Live RT4P cells were induced to undergo apoptosis and blebbishield formation and subjected to transformation (shown in the schematic of supplemental figure 1b on left). The transformation phase was subjected to time-lapse microscopy, which showed the exit phase, during which polarized cells emerged out of transformed spheres (orange arrows).
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MD Anderson's Cancer Center
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ARTICLE ABSTRACT
Genomic instability and immune evasion are hallmarks of cancer. Apoptotic cancer stem cells can evade cell death by undergoing cellular transformation by constructing “blebbishields” from apoptotic bodies. In this study, we report a novel linkage between genomic instability and phagocytosis evasion that is coordinated by the blebbishield emergency program. Blebbishield emergency program evaded genomic instability checkpoint, expressed genomic instability–associated genes at distinct phases of cellular transformation, exhibited chromosomal instability, and promoted increase in nuclear size. Blebbishields fused with immune cells to evade phagocytosis, and the resultant hybrid cells exhibited increased migration, tumorigenesis, metastasis, red blood cell recruitment to tumors, and induced hepatosplenomegaly with signatures of genomic instability, blebbishield emergency program, and phagocytosis evasion to offer poor prognosis. Overall, our data demonstrate that the blebbishield emergency program drives evasion of chromosomal instability and phagocytosis checkpoints by apoptotic cancer stem cells. Cancer Res; 77(22); 6144–56. ©2017 AACR.Usage metrics
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BiomarkersCell Death And SenescenceIntercellular interactions in apoptosisTranscriptional control of apoptosisComputational MethodsGene expression profilingDrug MechanismsCellular responses to anticancer drugsGenitourinary CancersBladder cancerImmunologyImmune responses to cancerTumor resistance to immune responseOncogenes & Tumor Suppressorsp53Stem Cell Biology
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