00085472can171546-sup-183813_4_video_4436497_p0lcb8.mp4 (1.3 MB)
Supplemental Movie 2: Interstitial convection, vascular perfusion and the location of blood vessels in an LS174T tumor from Investigating Low-Velocity Fluid Flow in Tumors with Convection-MRI
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posted on 2023-03-31, 01:25 authored by Simon Walker-Samuel, Thomas A. Roberts, Rajiv Ramasawmy, Jake S. Burrell, Sean Peter Johnson, Bernard M. Siow, Simon Richardson, Miguel R. Gonçalves, Douglas Pendse, Simon P. Robinson, R. Barbara Pedley, Mark F. LythgoeSupplemental Movie 2: Animated EVAC streamlines (grey lines), showing the path taken by interstitial fluid, overlaid on vascular perfusion measurements (colour scale, measured in vivo using arterial spin labelling) and the location of blood vessels (yellow structures, measured ex vivo using micro-CT). The data were acquired in an LS174T colorectal carcinoma tumour xenograft.
Funding
BBSRC/AstraZeneca Industrial Partnership Studentship
UK Regenerative Medicine Platform Safety Hub
Eli Lilly and Company
EPSRC
Wellcome Trust Senior Fellowship
Wellcome Trust
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ARTICLE ABSTRACT
Several distinct fluid flow phenomena occur in solid tumors, including intravascular blood flow and interstitial convection. Interstitial fluid pressure is often raised in solid tumors, which can limit drug delivery. To probe low-velocity flow in tumors resulting from raised interstitial fluid pressure, we developed a novel MRI technique named convection-MRI, which uses a phase-contrast acquisition with a dual-inversion vascular nulling preparation to separate intra- and extravascular flow. Here, we report the results of experiments in flow phantoms, numerical simulations, and tumor xenograft models to investigate the technical feasibility of convection-MRI. We observed a significant correlation between estimates of effective fluid pressure from convection-MRI with gold-standard, invasive measurements of interstitial fluid pressure in mouse models of human colorectal carcinoma. Our results show how convection-MRI can provide insights into the growth and responsiveness to vascular-targeting therapy in colorectal cancers.Significance: A noninvasive method for measuring low-velocity fluid flow caused by raised fluid pressure can be used to assess changes caused by therapy. Cancer Res; 78(7); 1859–72. ©2018 AACR.Usage metrics
Keywords
Drug ResistanceDrug transport and metabolismGastrointestinal CancersColorectal cancerImagingMagnetic resonance imaging (MRI)Small animal imagingPreclinical ModelsAnimal models of cancerProgression, Invasion & MetastasisImaging of tumor progression and metastasisInflammation and tumor developmentTumor MicroenvironmentTumor microcirculation
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