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Table S1, Table S2, Table S3, Table S4, Table S5, Table S6, Table S7, Table S8, Table S9, Table S10, Table S11, Figure S1, Figure S2, Figure S3, Text from Serum miRNA–based Prediction of Axillary Lymph Node Metastasis in Breast Cancer

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posted on 2023-03-31, 21:03 authored by Sho Shiino, Juntaro Matsuzaki, Akihiko Shimomura, Junpei Kawauchi, Satoko Takizawa, Hiromi Sakamoto, Yoshiaki Aoki, Masayuki Yoshida, Kenji Tamura, Ken Kato, Takayuki Kinoshita, Yuko Kitagawa, Takahiro Ochiya

Table S1. Definition criteria of categorical variables for logistic-LASSO regression analysis Table S2. Clinicopathological characteristics of patients with benign breast diseases Table S3. Clinicopathological characteristics of patients stratified into the training set and test set Table S4. Comparison of T staging between pre- and post-operative diagnosis Table S5. Comparison of lymphovascular invasion (LVI) between pre- and post-operative diagnosis Table S6. Clinicopathological characteristics of N-positive patients misdiagnosed as N-negative in the test set Table S7. Sensitivity and specificity analysis for the diagnostic index in the test set Table S8. Biomarker candidate miRNAs for N positivity Table S9. Comparison of post-operative lymphovascular invasion (LVI) between original and re-evaluated diagnosis in the training set Table S10. Characteristics of ultrasound findings of axillary lymph node status Table S11. Utility of ultrasonography for the detection of axillary lymph node metastasis Figure S1. Ultrasound findings of suspicious metastatic and negative axillary lymph nodes. Figure S2. Serum levels of miR-629-3p and miR-4710 in the training set. P-values were calculated by one-way ANOVA with Tukey's post-hoc analysis. Figure S3. ROC curves for histological grade and Ki-67 labeling index in the test set (postoperative pathology). Numbers in parentheses are 95% confidence intervals. Text. Materials and Methods

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NCC

National Cancer Center

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ARTICLE ABSTRACT

Sentinel lymph node biopsy (SLNB) is the gold-standard procedure for evaluating axillary lymph node (ALN) status in patients with breast cancer. However, the morbidity of SLNB is not negligible, and the procedure is invasive for patients without ALN metastasis. Here, we developed a diagnostic model for evaluating ALN status using a combination of serum miRNAs and clinicopathologic factors as a novel less-invasive biomarker.Experimental Design: Preoperative serum samples were collected from patients who underwent surgery for primary breast cancer or breast benign diseases between 2008 and 2014. A total of 958 serum samples (921 cases of primary breast cancer, including 630 cases in the no ALN metastasis group and 291 cases in the ALN metastasis group, and 37 patients with benign breast diseases) were analyzed by miRNA microarray. Samples were randomly divided into training and test sets. Logistic LASSO regression analysis was used to construct diagnostic models in the training set, which were validated in the test set. An optimal diagnostic model was identified using a combination of two miRNAs (miR-629-3p and miR-4710) and three clinicopathologic factors (T stage, lymphovascular invasion, and ultrasound findings), which showed a sensitivity of 0.88 (0.84–0.92), a specificity of 0.69 (0.61–0.76), an accuracy of 0.818, and an area under the receiver operating characteristic curve of 0.86 in the test set. Serum miRNA profiles may be useful for the diagnosis of ALN metastasis before surgery in a less-invasive manner than SLNB.

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