American Association for Cancer Research
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Table S10 from Fusobacterium Nucleatum Promotes the Development of Colorectal Cancer by Activating a Cytochrome P450/Epoxyoctadecenoic Acid Axis via TLR4/Keap1/NRF2 Signaling

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journal contribution
posted on 2023-03-31, 04:25 authored by Cheng Kong, Xuebing Yan, Yefei Zhu, Huiyuan Zhu, Ying Luo, Peipei Liu, Sylvain Ferrandon, Matthew F. Kalady, Renyuan Gao, Jide He, Fang Yin, Xiao Qu, Jiayi Zheng, Yaohui Gao, Qing Wei, Yanlei Ma, Jun-Yan Liu, Huanlong Qin

Table S10 shows the linoleic acid dietary composition.


National Nature Science Foundation of China

Emerging Cutting-Edge Technology Joint Research projects of Shanghai

Tongji University Subject Pilot Program

Special Construction of Integrated Traditional Chinese Medicine and Western Medicine in Shanghai General Hospital

Social Development Project of Yangzhou science and technology bureau

Program of Jiangsu Commission of Health



Emerging research has revealed regulation of colorectal cancer metabolism by bacteria. Fusobacterium nucleatum (Fn) plays a crucial role in the development of colorectal cancer, however, whether Fn infection modifies metabolism in patients with colorectal cancer remains unknown. Here, LC-MS/MS-based lipidomics identified the upregulation of cytochrome P450 monooxygenases, primarily CYP2J2, and their mediated product 12,13-EpOME in patients with colorectal cancer tumors and mouse models, which increased the invasive and migratory ability of colorectal cancer cells in vivo and in vitro by regulating the epithelial–mesenchymal transition (EMT). Metagenomic sequencing indicated a positive correlation between increased levels of fecal Fn and serum 12,13-EpOME in patients with colorectal cancer. High levels of CYP2J2 in tumor tissues also correlated with high Fn levels and worse overall survival in patients with stage III/IV colorectal cancer. Moreover, Fn was found to activate TLR4/AKT signaling, downregulating Keap1 and increasing NRF2 to promote transcription of CYP2J2. Collectively, these data identify that Fn promotes EMT and metastasis in colorectal cancer by activating a TLR4/Keap1/NRF2 axis to increase CYP2J2 and 12,13-EpOME, which could serve as clinical biomarkers and therapeutic targets for Fn-infected patients with colorectal cancer. This study uncovers a mechanism by which Fusobacterium nucleatum regulates colorectal cancer metabolism to drive metastasis, suggesting the potential biomarker and therapeutic utility of the CYP2J2/12,13–EpOME axis in Fn-infected patients.

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