15357163mct180918-sup-206985_2_supp_5389106_p426kj.docx (2.5 MB)
Supplementary methods and Supplementary Figures S1-S8 from BI1071, a Novel Nur77 Modulator, Induces Apoptosis of Cancer Cells by Activating the Nur77-Bcl-2 Apoptotic Pathway
journal contribution
posted on 2023-04-03, 15:02 authored by Xiaohui Chen, Xihua Cao, Xuhuang Tu, Gulimiran Alitongbieke, Zebin Xia, Xiaotong Li, Ziwen Chen, Meimei Yin, Dan Xu, Shangjie Guo, Zongxi Li, Liqun Chen, Xindao Zhang, Dingyu Xu, Meichun Gao, Jie Liu, Zhiping Zeng, Hu Zhou, Ying Su, Xiao-kun ZhangThe Supplementary Methods show the Synthesis of DIM-C-pPhCF3+MeSO3- (BI1071); Figure S1 shows BI1071 induces apoptosis in different cancer cell lines; Fig. S2 shows BI1071 induces apoptosis in different breast cancer cell lines; Fig. S3 shows BI1071 induces apoptosis in cancer cell lines but not in non-transformed cell lines; Fig. S4 shows BI1071 did't induce the body weight loss; Fi.g S5 shows overexpression of Nur77-LBD enhances the killing effect of BI1071; Fig. S6 shows BI1071 has no effect on other nuclear receptors; Fig. S7 shows the imparied effect of BI1071 could be rescued by re-expression of Bcl-2; Fig. S8 shows BI1071 binding to Nur77 promotes Nur77 interaction with Bcl-2 and mitochondrial localization