American Association for Cancer Research
Browse
23266066cir150221-sup-155026_3_supp_3406872_b45fr4.docx (182.56 kB)

Supplementary Tables 1 and 2 and Supplementary Figures 1 and 2 from Antitumor Efficacy of Anti-GD2 IgG1 Is Enhanced by Fc Glyco-Engineering

Download (182.56 kB)
journal contribution
posted on 2023-04-03, 23:02 authored by Hong Xu, Hongfen Guo, Irene Y. Cheung, Nai-Kong V. Cheung

Supplemental Table S1: Affinities relative to hu3F8-IgG1. Supplemental Table S2: Affinities to both human and mouse FcR(n). Supplemental Figure S1: Kinetic analysis of in vitro binding of antibodies to CD16A(158V) by SPR. Supplemental Figure S2: Blood clearance of the antibodies in nude mice.

Funding

the Band of Parents, Arms Wide Open Cancer Foundation, Kids Walk for Kids with Cancer NYC, Robert Steel Foundation, and Enid A. Haupt Chair

NIH

History

ARTICLE ABSTRACT

The affinity of therapeutic antibodies for Fcγ receptors (FcγRs) strongly influences their antitumor potency. To generate antibodies with optimal binding and immunologic efficacy, we compared the affinities of different versions of an IgG1 Fc region that had an altered peptide backbone, altered glycans, or both. To produce IgG1 with glycans that lacked α1,6-fucose, we used CHO cells that were deficient in the enzyme UDP-N-acetylglucosamine: α-3-d-mannoside-β-1,2-N-acetylglucosaminyltransferase I (GnT1), encoded by the MGAT1 gene. Mature N-linked glycans require this enzyme, and without it, CHO cells synthesize antibodies carrying only Man5-GlcNAc2, which were more effective in antibody-dependent cell-mediated cytotoxicity (ADCC). Our engineered IgG1, hu3F8-IgG1, is specific for GD2, a neuroendocrine tumor ganglioside. Its peptide mutant is IgG1-DEL (S239D/I332E/A330L), both produced in wild-type CHO cells. When produced in GnT1-deficient CHO cells, we refer to them as IgG1n and IgG1n-DEL, respectively. Affinities for human FcγRs were measured using Biacore T-100 (on CD16 and CD32 polymorphic alleles), their immunologic properties compared for ADCC and complement-mediated cytotoxicity (CMC) in vitro, and pharmacokinetics and antitumor effects were compared in vivo in humanized mice. IgG1n and IgG1n-DEL contained only mannose and acetylglucosamine and had preferential affinity for activating CD16s, over inhibitory CD32B, receptors. In vivo, the antitumor effects of IgG1, IgG1-DEL, and IgG1n-DEL were similar but modest, whereas IgG1n was significantly more effective (P < 0.05). Thus, IgG1n antibodies produced in GnT1-deficient CHO cells may have potential as improved anticancer therapeutics. Cancer Immunol Res; 4(7); 631–8. ©2016 AACR.

Usage metrics

    Cancer Immunology Research

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC