Supplementary Table 5. from Accumulation of Circulating CCR7+ Natural Killer Cells Marks Melanoma Evolution and Reveals a CCL19-Dependent Metastatic Pathway

Cristiani, Costanza Maria; Turdo, Alice; Ventura, Valeria; Apuzzo, Tiziana; Capone, Mariaelena; Madonna, Gabriele; Mallardo, Domenico; Garofalo, Cinzia; Giovannone, Emilia Dora; Grimaldi, Antonio M.; Tallerico, Rossana; Marcenaro, Emanuela; Pesce, Silvia; Del Zotto, Genny; Agosti, Valter; Costanzo, Francesco Saverio; Gulletta, Elio; Rizzo, Aroldo; Moretta, Alessandro; Karre, Klas; Ascierto, Paolo A.; Todaro, Matilde; Carbone, Ennio

doi:10.1158/2326-6066.22542756.v1

23266066cir180651-sup-208946_2_supp_5326446_pmh9x9.pdf (50.92 kB)

Supplementary Table 5. from Accumulation of Circulating CCR7⁺ Natural Killer Cells Marks Melanoma Evolution and Reveals a CCL19-Dependent Metastatic Pathway

journal contribution

posted on 2023-04-04, 00:45 authored by Costanza Maria Cristiani, Alice Turdo, Valeria Ventura, Tiziana Apuzzo, Mariaelena Capone, Gabriele Madonna, Domenico Mallardo, Cinzia Garofalo, Emilia Dora Giovannone, Antonio M. Grimaldi, Rossana Tallerico, Emanuela Marcenaro, Silvia Pesce, Genny Del Zotto, Valter Agosti, Francesco Saverio Costanzo, Elio Gulletta, Aroldo Rizzo, Alessandro Moretta, Klas Karre, Paolo A. Ascierto, Matilde Todaro, Ennio Carbone

Univariate analysis sera cytokines from melanoma patients.

Funding

University of Genoa and Istituto Giannina Gaslini, Genoa

Italian Association for Cancer Research

History

ARTICLE ABSTRACT

Immune checkpoint blockade therapy has changed prognoses for many melanoma patients. However, immune responses that correlate with clinical progression of the disease are still poorly understood. To identify immune responses correlating with melanoma clinical evolution, we analyzed serum cytokines as well as circulating NK and T-cell subpopulations from melanoma patients. The patients' immune profiles suggested that melanoma progression leads to changes in peripheral blood NK and T-cell subsets. Stage IV melanoma was characterized by an increased frequency of CCR7+CD56bright NK cells as well as high serum concentrations of the CCR7 ligand CCL19. CCR7 expression and CCL19 secretion were also observed in melanoma cell lines. The CCR7+ melanoma cell subpopulation coexpressed PD-L1 and Galectin-9 and had stemness properties. Analysis of melanoma-derived cancer stem cells (CSC) showed high CCR7 expression; these CSCs were efficiently recognized and killed by NK cells. An accumulation of CCR7+, PD-L1+, and Galectin-9+ melanoma cells in melanoma metastases was demonstrated ex vivo. Altogether, our data identify biomarkers that may mark a CCR7-driven metastatic melanoma pathway.

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Keywords

Computational Methods Software Immunology Immune responses to cancer Immunotherapy Cellular immunotherapy Progression, Invasion & Metastasis Skin Cancers Melanoma

Licence

CC BY