American Association for Cancer Research
00085472can130530-sup-table_2.pdf (58.74 kB)

Supplementary Table 2 from Adipocyte-Derived Fibroblasts Promote Tumor Progression and Contribute to the Desmoplastic Reaction in Breast Cancer

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journal contribution
posted on 2023-03-30, 21:49 authored by Ludivine Bochet, Camille Lehuédé, Stéphanie Dauvillier, Yuan Yuan Wang, Béatrice Dirat, Victor Laurent, Cédric Dray, Romain Guiet, Isabelle Maridonneau-Parini, Sophie Le Gonidec, Bettina Couderc, Ghislaine Escourrou, Philippe Valet, Catherine Muller

PDF file, 58K, Sequences and concentrations of the primers used to detect human and murine genes expression by qPCR.



Cancer-associated fibroblasts (CAF) comprise the majority of stromal cells in breast cancers, yet their precise origins and relative functional contributions to malignant progression remain uncertain. Local invasion leads to the proximity of cancer cells and adipocytes, which respond by phenotypical changes to generate fibroblast-like cells termed as adipocyte-derived fibroblasts (ADF) here. These cells exhibit enhanced secretion of fibronectin and collagen I, increased migratory/invasive abilities, and increased expression of the CAF marker FSP-1 but not α-SMA. Generation of the ADF phenotype depends on reactivation of the Wnt/β-catenin pathway in response to Wnt3a secreted by tumor cells. Tumor cells cocultivated with ADFs in two-dimensional or spheroid culture display increased invasive capabilities. In clinical specimens of breast cancer, we confirmed the presence of this new stromal subpopulation. By defining a new stromal cell population, our results offer new opportunities for stroma-targeted therapies in breast cancer. Cancer Res; 73(18); 5657–68. ©2013 AACR.

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