- No file added yet -
Supplementary Table 1 from miRNA-302b Suppresses Human Hepatocellular Carcinoma by Targeting AKT2
journal contribution
posted on 2023-04-03, 16:02 authored by Lumin Wang, Jiayi Yao, Xiaogang Zhang, Bo Guo, Xiaofeng Le, Mark Cubberly, Zongfang Li, Kejun Nan, Tusheng Song, Chen HuangPDF file - 42K, The relationship between miR-302b levels and differentiated tumor.
History
ARTICLE ABSTRACT
miRNAs (miR) play a critical role in human cancers, including hepatocellular carcinoma. Although miR-302b has been suggested to function as a tumor repressor in other cancers, its role in hepatocellular carcinoma is unknown. This study investigated the expression and functional role of miR-302b in human hepatocellular carcinoma. The expression level of miR-302b is dramatically decreased in clinical hepatocellular carcinoma specimens, as compared with their respective nonneoplastic counterparts, and in hepatocellular carcinoma cell lines. Overexpression of miR-302b suppressed hepatocellular carcinoma cell proliferation and G1–S transition in vitro, whereas inhibition of miR-302b promoted hepatocellular carcinoma cell proliferation and G1–S transition. Using a luciferase reporter assay, AKT2 was determined to be a direct target of miR-302b. Subsequent investigation revealed that miR-302b expression was inversely correlated with AKT2 expression in hepatocellular carcinoma tissue samples. Importantly, silencing AKT2 recapitulated the cellular and molecular effects seen upon miR-302b overexpression, which included inhibiting hepatocellular carcinoma cell proliferation, suppressing G1 regulators (Cyclin A, Cyclin D1, CDK2) and increasing p27Kip1 phosphorylation at Ser10. Restoration of AKT2 counteracted the effects of miR-302b expression. Moreover, miR-302b was able to repress tumor growth of hepatocellular carcinoma cells in vivo.Implications: Taken together, miR-302b inhibits HCC cell proliferation and growth in vitro and in vivo by targeting AKT2. Mol Cancer Res; 12(2); 190–202. ©2013 AACR.Usage metrics
Categories
Keywords
Cell CycleControl of cell cycle progressionSignal transduction pathwaysCell SignalingDrug TargetsOncoprotein & tumor suppressor drug targetsGastrointestinal CancersLiver cancerGenome BiologySilencing and reactivation of gene expressionOncogenes & Tumor SuppressorsTumor suppressor genesPreclinical ModelsAnimal models of cancer
Licence
Exports
RefWorksRefWorks
BibTeXBibTeX
Ref. managerRef. manager
EndnoteEndnote
DataCiteDataCite
NLMNLM
DCDC