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00085472can130022-sup-tab1.pdf (369.95 kB)

Supplementary Table 1 from Intestinal Bacteria Modify Lymphoma Incidence and Latency by Affecting Systemic Inflammatory State, Oxidative Stress, and Leukocyte Genotoxicity

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posted on 2023-03-30, 21:48 authored by Mitsuko L. Yamamoto, Irene Maier, Angeline Tilly Dang, David Berry, Jared Liu, Paul M. Ruegger, Jiue-in Yang, Phillip A. Soto, Laura L. Presley, Ramune Reliene, Aya M. Westbrook, Bo Wei, Alexander Loy, Christopher Chang, Jonathan Braun, James Borneman, Robert H. Schiestl

PDF file - 369K, Reverse PCR primers used in the Illumina-based high throughput sequence analysis of bacterial rRNA genes.

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ARTICLE ABSTRACT

Ataxia-telangiectasia is a genetic disorder associated with high incidence of B-cell lymphoma. Using an ataxia-telangiectasia mouse model, we compared lymphoma incidence in several isogenic mouse colonies harboring different bacterial communities, finding that intestinal microbiota are a major contributor to disease penetrance and latency, lifespan, molecular oxidative stress, and systemic leukocyte genotoxicity. High-throughput sequence analysis of rRNA genes identified mucosa-associated bacterial phylotypes that were colony-specific. Lactobacillus johnsonii, which was deficient in the more cancer-prone mouse colony, was causally tested for its capacity to confer reduced genotoxicity when restored by short-term oral transfer. This intervention decreased systemic genotoxicity, a response associated with reduced basal leukocytes and the cytokine-mediated inflammatory state, and mechanistically linked to the host cell biology of systemic genotoxicity. Our results suggest that intestinal microbiota are a potentially modifiable trait for translational intervention in individuals at risk for B-cell lymphoma, or for other diseases that are driven by genotoxicity or the molecular response to oxidative stress. Cancer Res; 73(14); 4222–32. ©2013 AACR.

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