American Association for Cancer Research
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10559965epi110221-sup-epi-11-0221r_chaturvedi_supp_table_1.pdf (150.65 kB)

Supplementary Table 1 from Evaluation of Multiplexed Cytokine and Inflammation Marker Measurements: a Methodologic Study

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posted on 2023-03-31, 13:20 authored by Anil K. Chaturvedi, Troy J. Kemp, Ruth M. Pfeiffer, Angelique Biancotto, Marcus Williams, Stella Munuo, Mark P. Purdue, Ann W. Hsing, Ligia Pinto, J. Philip McCoy, Allan Hildesheim
Supplementary Table 1 from Evaluation of Multiplexed Cytokine and Inflammation Marker Measurements: a Methodologic Study

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ARTICLE ABSTRACT

Background: Chronic inflammation is etiologically related to several cancers. We evaluated the performance [ability to detect concentrations above the assay's lower limit of detection, coefficients of variation (CV), and intraclass correlation coefficients (ICC)] of 116 inflammation, immune, and metabolic markers across two Luminex bead–based commercial kits and three specimen types.Methods: From 100 cancer-free participants in the Prostate, Lung, Colorectal, and Ovarian Cancer Trial, serum, heparin plasma, and EDTA plasma samples were utilized. We measured levels of 67 and 97 markers using Bio-Rad and Millipore kits, respectively. Reproducibility was assessed using 40 blinded duplicates (20 within-batches and 20 across-batches) for each specimen type.Results: A majority of markers were detectable in more than 25% of individuals on all specimen types/kits. Of the 67 Bio-Rad markers, 51, 52, and 47 markers in serum, heparin plasma, and EDTA plasma, respectively, had across-batch CVs of less than 20%. Likewise, of 97 Millipore markers, 75, 69, and 78 markers in serum, heparin plasma, and EDTA plasma, respectively, had across-batch CVs of less than 20%. When results were combined across specimen types, 45 Bio-Rad and 71 Millipore markers had acceptable performance (>25% detectability on all three specimen types and across-batch CVs <20% on at least two of three specimen types). Median concentrations and ICCs differed to a small extent across specimen types and to a large extent between Bio-Rad and Millipore.Conclusions: Inflammation and immune markers can be measured reliably in serum and plasma samples using multiplexed Luminex-based methods.Impact: Multiplexed assays can be utilized for epidemiologic investigations into the role of inflammation in cancer etiology. Cancer Epidemiol Biomarkers Prev; 20(9); 1902–11. ©2011 AACR.

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