Supplementary Table 1 from Development and Clinical Validation of a Blood Test Based on 29-Gene Expression for Early Detection of Colorectal Cancer

Ciarloni, Laura; Ehrensberger, Sahar Hosseinian; Imaizumi, Natsuko; Monnier-Benoit, Sylvain; Nichita, Cristina; Myung, Seung-Jae; Kim, Joo Sung; Song, Si Young; Kim, Tae Il; van der Weg, Boudewijn; Meier, Rémy; Borovicka, Jan; Beglinger, Christoph; Vallet, Cédric; Maerten, Philippe; Rüegg, Curzio; Dorta, Gian

doi:10.1158/1078-0432.22455491.v1

Supplementary Table 1 from Development and Clinical Validation of a Blood Test Based on 29-Gene Expression for Early Detection of Colorectal Cancer

journal contribution

posted on 2023-03-31, 18:12 authored by Laura Ciarloni, Sahar Hosseinian Ehrensberger, Natsuko Imaizumi, Sylvain Monnier-Benoit, Cristina Nichita, Seung-Jae Myung, Joo Sung Kim, Si Young Song, Tae Il Kim, Boudewijn van der Weg, Rémy Meier, Jan Borovicka, Christoph Beglinger, Cédric Vallet, Philippe Maerten, Curzio Rüegg, Gian Dorta

The 29-gene panel for colorectal cancer and large adenoma detection. Gene expression profiles obtained from the training set were explored by univariate analysis to compare the behavior of the 29 genes across the control (CON), the CRC and the LAP groups. Twelve genes were significantly differentially expressed in CRC and LAP compared to controls (p-value<0.05) by Wilcoxon rank test, applied to normalized gene expressions. Relative abundance of a gene transcript is represented by the fold change (FC), defined as ã€-FCã€-_gene ã€-=2ã€-^â^†â^†Cp , where â^†â^†Cp=mean(ã€-â^†Cpã€-_Disease )-mean(ã€-â^†Cpã€-_Control ).

Funding

Novigenix SA and Diagnoplex SA

History

ARTICLE ABSTRACT

Purpose: A blood test for early detection of colorectal cancer is a valuable tool for testing asymptomatic individuals and reducing colorectal cancer–related mortality. The objective of this study was to develop and validate a novel blood test able to differentiate patients with colorectal cancer and adenomatous polyps (AP) from individuals with a negative colonoscopy.Experimental Design: A case–control, multicenter clinical study was designed to collect blood samples from patients referred for colonoscopy or surgery. Predictive algorithms were developed on 75 controls, 61 large AP (LAP) ≥1 cm, and 45 colorectal cancer cases and independently validated on 74 controls, 42 LAP, and 52 colorectal cancer cases (23 stages I–II) as well as on 245 cases including other colorectal findings and diseases other than colorectal cancer. The test is based on a 29-gene panel expressed in peripheral blood mononuclear cells alone or in combination with established plasma tumor markers.Results: The 29-gene algorithm detected colorectal cancer and LAP with a sensitivity of 79.5% and 55.4%, respectively, with 90.0% specificity. Combination with the protein tumor markers carcinoembryonic antigen (CEA) and CYFRA21-2 resulted in a specificity increase (92.2%) with a sensitivity for colorectal cancer and LAP detection of 78.1% and 52.3%, respectively.Conclusions: We report the validation of a novel blood test, Colox®, for the detection of colorectal cancer and LAP based on a 29-gene panel and the CEA and CYFRA21-1 plasma biomarkers. The performance and convenience of this routine blood test provide physicians a useful tool to test average-risk individuals unwilling to undergo upfront colonoscopy. Clin Cancer Res; 22(18); 4604–11. ©2016 AACR.