Supplementary Table 1. Supplementary Figures 1 and 2. from Intratumoral Payload Concentration Correlates with the Activity of Antibody–Drug Conjugates

Zhang, Donglu; Yu, Shang-Fan; Khojasteh, S. Cyrus; Ma, Yong; Pillow, Thomas H.; Sadowsky, Jack D.; Su, Dian; Kozak, Katherine R.; Xu, Keyang; Polson, Andrew G.; Dragovich, Peter S.; Hop, Cornelis E.C.A.

doi:10.1158/1535-7163.22504461.v1

Supplementary Table 1. Supplementary Figures 1 and 2. from Intratumoral Payload Concentration Correlates with the Activity of Antibody–Drug Conjugates

https://doi.org/10.1158/1535-7163.22504461

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posted on 2023-04-03, 15:00 authored by Donglu Zhang, Shang-Fan Yu, S. Cyrus Khojasteh, Yong Ma, Thomas H. Pillow, Jack D. Sadowsky, Dian Su, Katherine R. Kozak, Keyang Xu, Andrew G. Polson, Peter S. Dragovich, Cornelis E.C.A. Hop

<p>Table S1. (A) Quantitation of PBD in tumors and (B) total antibody in plasma of xenograft mice following intravenously administration of ADC conjugates. Figure S1. (A-D) Correlation (X-Y plots) of tumor growth inhibition with intratumor PBD exposures after intravenous administration of corresponding ADCs A1-A4. Figure S2. Disulfide stability and immolation for payload release in incubations of ADCs A1-A4 in the presence of cysteine at pH 5.5 or 7.0.</p>

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DOI - Is supplement to Intratumoral Payload Concentration Correlates with the Activity of Antibody–Drug Conjugates

ARTICLE ABSTRACT

Antibody–drug conjugates (ADC) have become important scaffolds for targeted cancer therapies. However, ADC exposure–response correlation is not well characterized. We demonstrated that intratumor payload exposures correlated well with the corresponding efficacies of several disulfide-linked ADCs, bearing an DNA alkylating agent, pyrrolo[2,1-c][1,4]benzodiazepine-dimer (PBD), in HER2-expressing xenograft models. The correlation suggests that a threshold concentration of intratumor payload is required to support sustained efficacy and an ADC can deliver an excessive level of payload to tumors that does not enhance efficacy (“Plateau” effect). In contrast to tumor PBD concentrations, related assessments of systemic exposures, plasma stability, and drug-to-antibody ratio changes of related ADCs did not consistently rationalize the observed ADC efficacies. A minimal efficacious dose could be determined by ADC dose-fractionation studies in the xenograft models. Mechanistic investigations revealed that both linker immolation and linker disulfide stability are the key factors that determine intratumor PBD concentrations. Overall, this study demonstrates how a linker design can impact ADC efficacy and that the intratumor exposure of a payload drug as the molecular mechanism quantitatively correlate with and predict the antitumor efficacy of ADCs. Mol Cancer Ther; 17(3); 677–85. ©2018 AACR.

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Cancer Prevention Biological mechanisms in prevention Drug Resistance Drug transport and metabolism Small Molecule Agents DNA-reactive agents

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Supplementary Table 1. Supplementary Figures 1 and 2. from Intratumoral Payload Concentration Correlates with the Activity of Antibody–Drug Conjugates

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