Supplementary Methods from Activation of the PD-1 Pathway Contributes to Immune Escape in EGFR-Driven Lung Tumors

Akbay, Esra A.; Koyama, Shohei; Carretero, Julian; Altabef, Abigail; Tchaicha, Jeremy H.; Christensen, Camilla L.; Mikse, Oliver R.; Cherniack, Andrew D.; Beauchamp, Ellen M.; Pugh, Trevor J.; Wilkerson, Matthew D.; Fecci, Peter E.; Butaney, Mohit; Reibel, Jacob B.; Soucheray, Margaret; Cohoon, Travis J.; Janne, Pasi A.; Meyerson, Matthew; Hayes, D. Neil; Shapiro, Geoffrey I.; Shimamura, Takeshi; Sholl, Lynette M.; Rodig, Scott J.; Freeman, Gordon J.; Hammerman, Peter S.; Dranoff, Glenn; Wong, Kwok-Kin

doi:10.1158/2159-8290.22530261.v1

21598290cd130310-sup-supp_methods.pdf (306.85 kB)

Supplementary Methods from Activation of the PD-1 Pathway Contributes to Immune Escape in EGFR-Driven Lung Tumors

journal contribution

posted on 2023-04-03, 20:45 authored by Esra A. Akbay, Shohei Koyama, Julian Carretero, Abigail Altabef, Jeremy H. Tchaicha, Camilla L. Christensen, Oliver R. Mikse, Andrew D. Cherniack, Ellen M. Beauchamp, Trevor J. Pugh, Matthew D. Wilkerson, Peter E. Fecci, Mohit Butaney, Jacob B. Reibel, Margaret Soucheray, Travis J. Cohoon, Pasi A. Janne, Matthew Meyerson, D. Neil Hayes, Geoffrey I. Shapiro, Takeshi Shimamura, Lynette M. Sholl, Scott J. Rodig, Gordon J. Freeman, Peter S. Hammerman, Glenn Dranoff, Kwok-Kin Wong

- PDF file 306K, MRI tumor volume quantification, lists for all the antibodies used in the experiments, gating method for Flow cytometry analysis, and supplementary references

History

ARTICLE ABSTRACT

The success in lung cancer therapy with programmed death (PD)-1 blockade suggests that immune escape mechanisms contribute to lung tumor pathogenesis. We identified a correlation between EGF receptor (EGFR) pathway activation and a signature of immunosuppression manifested by upregulation of PD-1, PD-L1, CTL antigen-4 (CTLA-4), and multiple tumor-promoting inflammatory cytokines. We observed decreased CTLs and increased markers of T-cell exhaustion in mouse models of EGFR-driven lung cancer. PD-1 antibody blockade improved the survival of mice with EGFR-driven adenocarcinomas by enhancing effector T-cell function and lowering the levels of tumor-promoting cytokines. Expression of mutant EGFR in bronchial epithelial cells induced PD-L1, and PD-L1 expression was reduced by EGFR inhibitors in non–small cell lung cancer cell lines with activated EGFR. These data suggest that oncogenic EGFR signaling remodels the tumor microenvironment to trigger immune escape and mechanistically link treatment response to PD-1 inhibition.Significance: We show that autochthonous EGFR-driven lung tumors inhibit antitumor immunity by activating the PD-1/PD-L1 pathway to suppress T-cell function and increase levels of proinflammatory cytokines. These findings indicate that EGFR functions as an oncogene through non–cell-autonomous mechanisms and raise the possibility that other oncogenes may drive immune escape. Cancer Discov; 3(12); 1355–63. ©2013 AACR.See related commentary by Rech and Vonderheide, p. 1330This article is highlighted in the In This Issue feature, p. 1317