Supplementary Methods, Table 1, Figures 1-6 from Expression of the p53 Target CDIP Correlates with Sensitivity to TNFα-Induced Apoptosis in Cancer Cells
posted on 2023-03-30, 21:26authored byLauren Brown-Endres, David Schoenfeld, Fang Tian, Hyung-Gu Kim, Takushi Namba, César Muñoz-Fontela, Anna Mandinova, Stuart A. Aaronson, Sam W. Lee
PDF file - 1.6MB, Table 1. Primers used for real-time PCR analysis of NF-kB target gene expression. Fig. S1. Recombinant human TNF�� rescues CDIP apoptosis in TNF�� knockdown cells. Fig. S2. TNF�� signaling was assessed in U2OS-CDIP cells 20 and 30 hours post-CDIP induction (dox), and 20 hours after treatment with 5 ng/mL recombinant human TNF�� (rhTNF��, boxed panel). Fig. S3. MAPK inhibitors do not block cell death induced by CDIP expression. Fig. S4. The NF-���� transcriptional program is altered in CDIP expressing cells. Fig. S5. The effects of IL-8 and ROS inhibition on CDIP��TNF�� apoptosis. Fig. S6. CDIP do not induce Fas.