Supplementary Methods S1 from Ultra High-plex Spatial Proteogenomic Investigation of Giant Cell Glioblastoma Multiforme Immune Infiltrates Reveals Distinct Protein and RNA Expression Profiles

Bonnett, Shilah A.; Rosenbloom, Alyssa B.; Ong, Giang T.; Conner, Mark; Rininger, Aric B.E.; Newhouse, Daniel; New, Felicia; Phan, Chi Q.; Ilcisin, Saskia; Sato, Hiromi; Lyssand, John S.; Geiss, Gary; Beechem, Joseph M.

doi:10.1158/2767-9764.22744279.v1

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Supplementary Methods S1 from Ultra High-plex Spatial Proteogenomic Investigation of Giant Cell Glioblastoma Multiforme Immune Infiltrates Reveals Distinct Protein and RNA Expression Profiles

journal contribution

posted on 2023-05-03, 14:20 authored by Shilah A. Bonnett, Alyssa B. Rosenbloom, Giang T. Ong, Mark Conner, Aric B.E. Rininger, Daniel Newhouse, Felicia New, Chi Q. Phan, Saskia Ilcisin, Hiromi Sato, John S. Lyssand, Gary Geiss, Joseph M. Beechem

Supplementary Methods S1: Protocol for proteogenomic workflow

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ARTICLE ABSTRACT

We describe ultra high-plex spatial proteogenomics; profiling whole transcriptome and high-plex proteomics on a single FFPE tissue section with spatial resolution. Investigation of gcGBM versus GBM revealed distinct protein and RNA expression profiles.