Supplementary Methods, Figures 1 - 9 from Elevation of Receptor Tyrosine Kinases by Small Molecule AKT Inhibitors in Prostate Cancer Is Mediated by Pim-1

PDF file - 1094K, Supplementary Methods including antibodies, PCR primers, constructs, 7-Methyl-GTP cap binding assay, Phospho-RTK arrays, and Methionine incorporation assay. Supplementary Figure 1. AKT inhibition induces expression of Pim-1 and RTKs. Supplementary Figure 2. GSK690693 induced RTK upregulation is blocked by a Pim kinase inhibitor in prostate cancer cells. Supplementary Figure 3. Phospho-RTK array analysis of cells treated with AKT and Pim inhibitors. Supplementary Figure 4. Pim kinase inhibitors reduce the expression of RTKs. Supplementary Figure 5. RTK levels are insensitive to mTOR inhibitors. Supplementary Figure 6. Pim-1 does not control RTK mRNA expression or protein half-life. Supplementary Figure 7. Knockdown of Pim-1 suppresses GSK690693-induced MET and IGF1R IRES activities. Supplementary Figure 8. GSK690693 and SMI-4a synergistically blocks the proliferation of DU145 cells. Supplementary Figure 9. Inhibition of protein translation by GSK690693 and PP242.