American Association for Cancer Research
00085472can133279-sup-can-13-3279methsfigs1-6tabs1-5.pdf (849.43 kB)

Supplementary Methods, Figures 1 - 6, Tables 1 - 5 from Identification of a Tumor-Suppressive Human-Specific MicroRNA within the FHIT Tumor-Suppressor Gene

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journal contribution
posted on 2023-03-30, 22:22 authored by Baocheng Hu, Xiaomin Ying, Jian Wang, Jittima Piriyapongsa, I. King Jordan, Jipo Sheng, Fang Yu, Po Zhao, Yazhuo Li, Hongyan Wang, Wooi Loon Ng, Shuofeng Hu, Xiang Wang, Chenguang Wang, Xiaofei Zheng, Wuju Li, Walter J. Curran, Ya Wang

PDF file - 858KB, Supplementary Methods, Supplementary References, Figure S1-6, Table S1-5. Figure S1. Identification of a new miR in FHIT intron. Figure S2. The SNP variant around the Pri-C2-miR region in FHIT did not affect the C2-miR expression. Figure S3. C2-miR and FHIT are under the same transcriptional control. Figure S4. C2-miR inhibiting human tumor cell growth was not through promoting apoptosis. Figure S5. C2-miR inhibits human tumor cell growth through targeting CCND1, ERBB2, DNMT3A or DNMT3B. Figure S6. Multiple members of the miR-548 family could target the same oncogenes. Table S1. The documented miR-548 family members. Table S2. Quantities of FHIT or C2-miR in total cell RNA (20 ng). Table S3. Expression comparison of pri-miRs or mature miRs in 293FT cells. Table S4. Primers used in this study. Table S5. NanoString probes for detecting 29 members of the miR-548 family.



Loss or attenuated expression of the tumor-suppressor gene FHIT is associated paradoxically with poor progression of human tumors. Fhit promotes apoptosis and regulates reactive oxygen species; however, the mechanism by which Fhit inhibits tumor growth in animals remains unclear. In this study, we used a multidisciplinary approach based on bioinformatics, small RNA library screening, human tissue analysis, and a xenograft mouse model to identify a novel member of the miR-548 family in the fourth intron of the human FHIT gene. Characterization of this human-specific microRNA illustrates the importance of this class of microRNAs in tumor suppression and may influence interpretation of Fhit action in human cancer. Cancer Res; 74(8); 2283–94. ©2014 AACR.