00085472can163144-sup-174843_3_supp_4166644_3t310g.pdf (103.6 kB)
Supplementary Materials and Methods from PRC2-Mediated Transcriptomic Alterations at the Embryonic Stage Govern Tumorigenesis and Clinical Outcome in MYCN-Driven Neuroblastoma
journal contribution
posted on 2023-03-31, 00:41 authored by Shoma Tsubota, Satoshi Kishida, Teppei Shimamura, Miki Ohira, Satoshi Yamashita, Dongliang Cao, Shinichi Kiyonari, Toshikazu Ushijima, Kenji KadomatsuThis file contains additional details of materials and methods used in this study including medium preparation, H&E staining, immunofluorescence analysis of frozen sections, immunohistochemistry on paraffin sections, in situ hybridization, western blotting, qPCR, microarray analysis, array CGH, MBD-seq, ChIP-seq, immunoprecipitation, overexpression of MYCN, knockdown of Ezh2, Ezh2 inhibitor treatment, and statistical analysis.