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Supplementary Materials and Methods from Mitochondria Transfer from Mesenchymal Stem Cells Confers Chemoresistance to Glioblastoma Stem Cells through Metabolic Rewiring

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posted on 2023-06-14, 14:20 authored by Jean Nakhle, Khattar Khattar, Tülin Özkan, Adel Boughlita, Daouda Abba Moussa, Amélie Darlix, Frédérique Lorcy, Valérie Rigau, Luc Bauchet, Sabine Gerbal-Chaloin, Martine Daujat-Chavanieu, Floriant Bellvert, Laurent Turchi, Thierry Virolle, Jean-Philippe Hugnot, Nicolas Buisine, Mireille Galloni, Valérie Dardalhon, Anne-Marie Rodriguez, Marie-Luce Vignais

TMZ dose-response assay

Caspase-GloⓇ 3/7 assay

Cytotox assay

Extracellular Flux Assays

Cell Proliferation

Western Blot

Flow Cytometry

Metabolite usage Mitoplates

Mass Spectrometry Quantification

13C stable isotope tracing experiments

Preparation and quantification of MSC mtDNA in GSCs

Imaging

Funding

Ligue Contre le Cancer (French League Against Cancer)

Fondation ARC pour la Recherche sur le Cancer (ARC)

Institut National Du Cancer (INCa)

Région Occitanie Pyrénées-Méditerranée

History

ARTICLE ABSTRACT

Mitochondria acquired from MSCs enhance the chemoresistance of GBMs. The discovery that they also generate metabolic vulnerability in GSCs paves the way for novel therapeutic approaches.

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    Cancer Research Communications

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    Keywords

    CNS CancersGliomas, GlioblastomasMetabolismMetabolomicsMitochondrial functionTumor MicroenvironmentTumor-stromal cell interactionsSmall Molecule AgentsAlkylating agentsDrug Resistance

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    CC BY

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