American Association for Cancer Research
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Supplementary Materials and Methods from Mitochondria Transfer from Mesenchymal Stem Cells Confers Chemoresistance to Glioblastoma Stem Cells through Metabolic Rewiring

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journal contribution
posted on 2023-06-14, 14:20 authored by Jean Nakhle, Khattar Khattar, Tülin Özkan, Adel Boughlita, Daouda Abba Moussa, Amélie Darlix, Frédérique Lorcy, Valérie Rigau, Luc Bauchet, Sabine Gerbal-Chaloin, Martine Daujat-Chavanieu, Floriant Bellvert, Laurent Turchi, Thierry Virolle, Jean-Philippe Hugnot, Nicolas Buisine, Mireille Galloni, Valérie Dardalhon, Anne-Marie Rodriguez, Marie-Luce Vignais
<p>TMZ dose-response assay</p><p>Caspase-GloⓇ 3/7 assay</p><p>Cytotox assay</p><p>Extracellular Flux Assays</p><p>Cell Proliferation</p><p>Western Blot</p><p>Flow Cytometry</p><p>Metabolite usage Mitoplates</p><p>Mass Spectrometry Quantification</p><p>13C stable isotope tracing experiments</p><p>Preparation and quantification of MSC mtDNA in GSCs</p><p>Imaging</p>

Funding

Ligue Contre le Cancer (French League Against Cancer)

Fondation ARC pour la Recherche sur le Cancer (ARC)

Institut National Du Cancer (INCa)

Région Occitanie Pyrénées-Méditerranée

History

ARTICLE ABSTRACT

Mitochondria acquired from MSCs enhance the chemoresistance of GBMs. The discovery that they also generate metabolic vulnerability in GSCs paves the way for novel therapeutic approaches.