American Association for Cancer Research
00085472can182900-sup-208793_2_supp_5365334_pnqzpl.docx (28.02 kB)

Supplementary Material and Methods from SATB2-AS1 Suppresses Colorectal Carcinoma Aggressiveness by Inhibiting SATB2-Dependent Snail Transcription and Epithelial–Mesenchymal Transition

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journal contribution
posted on 2023-03-31, 02:20 authored by Yi-Qing Wang, Dong-Mei Jiang, Sha-Sha Hu, Li Zhao, Lan Wang, Min-Hui Yang, Mei-Ling Ai, Hui-Juan Jiang, Yue Han, Yan-Qing Ding, Shuang Wang

Supplementary material and methods described the details, including cell culture, in situ hybridization, cell proliferation and colony formation assay, wound healing assay, cell invasion assays, western blot, RIP and ChIP assays, co-immunoprecipitation, and luciferase reporter assay.


National Basic Research Program of China

National Natural Science Foundation of China

Natural Science Foundation of Guangdong Province



Accumulating evidence suggests that long noncoding RNA (lncRNA) plays important regulatory roles in cancer biology. However, the involvement of lncRNA in colorectal carcinoma progression remains largely unknown, especially in colorectal carcinoma metastasis. In this study, we investigated the changes in lncRNA expression in colorectal carcinoma and identified a new lncRNA, the antisense transcript of SATB2 (SATB2-AS1), as a key regulator of colorectal carcinoma progression. SATB2-AS1 was frequently downregulated in colorectal carcinoma cells and tissues, and patients whose tumors expressed SATB2-AS1 at low levels had a shorter overall survival and poorer prognosis. Downregulation of SATB2-AS1 significantly promoted cell proliferation, migration, and invasion in vitro and in vivo, demonstrating that it acts as a tumor suppressor in colorectal carcinoma. SATB2-AS1 suppressed colorectal carcinoma progression by serving as a scaffold to recruit p300, whose acetylation of H3K27 and H3K9 at the SATB2 promoter upregulated expression of SATB2, a suppressor of colorectal carcinoma growth and metastasis. SATB2 subsequently recruited HDAC1 to the Snail promoter, repressing Snail transcription and inhibiting epithelial-to-mesenchymal transition. Taken together, these data reveal SATB2-AS1 as a novel regulator of the SATB2-Snail axis whose loss facilitates progression of colorectal carcinoma. These data show that the lncRNA SATB2-AS1 mediates epigenetic regulation of SATB2 and Snail expression to suppress colorectal cancer progression.See related commentary by Li, p. 3536