Supplementary Figures from Tumor Microenvironmental Conversion of Natural Killer Cells into Myeloid-Derived Suppressor Cells

Park, Young-Jun; Song, Boyeong; Kim, Yun-Sun; Kim, Eun-Kyung; Lee, Jung-Mi; Lee, Ga-Eun; Kim, Jae-Ouk; Kim, Yeon-Jeong; Chang, Woo-Sung; Kang, Chang-Yuil

doi:10.1158/0008-5472.22397915.v1

- No file added yet -

Supplementary Figures from Tumor Microenvironmental Conversion of Natural Killer Cells into Myeloid-Derived Suppressor Cells

journal contribution

posted on 2023-03-30, 21:52 authored by Young-Jun Park, Boyeong Song, Yun-Sun Kim, Eun-Kyung Kim, Jung-Mi Lee, Ga-Eun Lee, Jae-Ouk Kim, Yeon-Jeong Kim, Woo-Sung Chang, Chang-Yuil Kang

PDF file, 1963K, Inverse relationship between the percentage of Ly6Cneg/low cells and MDSCs (S1); Ly6Cneg/low cells are converted into MDSCs (S2); Only tumor CD49b+ cells, not CD4+ or CD8+ T cells or B cells, are converted into MDSCs (S3); 4 populations of conventional NK cells divided by maturation state (S4); The efficiency of conversion is increased in CD11bhighCD27high NKp46+ cells compared to NKp46+ total cells (S5); Endogenous MDSCs have no impact on NK cell death during the maturation course (S6).

History

ARTICLE ABSTRACT

How myeloid-derived suppressor cells (MDSC) emerge in the tumor environment remains unclear. Here, we report that GM-CSF can convert natural killer (NK) cells into MDSCs. When transferred into tumor-bearing mice, adoptively transferred NK cells lost their NK phenotype and were converted into Ly6ChighLy6Ghigh MDSC. This conversion was abolished by exposure to IL-2 either in vitro or in vivo. Notably, we found that of the 4 maturation stages based on CD11b/CD27 expression levels, only the CD11bhighCD27high NK cells could be converted into CD11b+Gr1+ MDSC ex vivo. Transfer of CD27high NK cells from tumor-bearing mice into tumor-bearing recipients was associated with conversion to MDSC in a manner associated with reduced numbers of CD11bhighCD27high and CD11bhighCD27low NK cell populations in the recipients. Our results identify a pathway of MDSC development from immature NK cells in tumor-bearing hosts, providing new insights into how tumor cells modulate their host immune microenvironment to escape immune surveillance. Cancer Res; 73(18); 5669–81. ©2013 AACR.