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Supplementary Figures and Tables from LncRNA AK036396 Inhibits Maturation and Accelerates Immunosuppression of Polymorphonuclear Myeloid–Derived Suppressor Cells by Enhancing the Stability of Ficolin B

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posted on 2023-04-04, 00:44 authored by Xinyu Tian, Yu Zheng, Kai Yin, Jie Ma, Jie Tian, Yue Zhang, Lingxiang Mao, Huaxi Xu, Shengjun Wang

Supplementary figures 1-6 and supplementary tables 1 and 2

Funding

Jiangsu Province's Key Medical Talents Program

Jiangsu Province

National Natural Science Foundation of China

Natural Science Foundation of Jiangsu

Jiangsu Planned Projects for Postdoctoral Research Funds

China's Post-doctoral Science Fund

History

ARTICLE ABSTRACT

Long noncoding RNAs (lncRNA) are emerging as crucial regulators of cell biology. However, the role of lncRNAs in the development and function of polymorphonuclear myeloid-derived suppressor cells (PMN-MDSC) remains unclear. Here, we identified that the lncRNA F730016J06Rik (AK036396) was highly expressed in PMN-MDSCs and that lncRNA AK036396 knockdown promoted the maturation and decreased the suppressive function of PMN-MDSCs. Ficolin B (Fcnb), the expression of which could be assessed as a surrogate for PMN-MDSC development, was the predicted target gene of lncRNA AK036396 based on microarray results. LncRNA AK036396 knockdown attenuated Fcnb protein stability in a manner dependent on the ubiquitin-proteasome system. Moreover, Fcnb inhibition downregulated the suppressive function of PMN-MDSCs. In addition, the expression of human M-ficolin, which is an ortholog of mouse Fcnb, was increased and positively correlated with arginase1 (ARG1) expression. This suppressive molecule is released by MDSCs, and its production is commonly used to represent the suppressive activity of MDSCs in patients with lung cancer, suggesting clinical relevance for these findings. These results indicate that lncRNA AK036396 can inhibit maturation and accelerate immunosuppression of PMN-MDSCs by enhancing Fcnb protein stability.

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