American Association for Cancer Research
15357163mct200547-sup-246378_3_supp_7266705_qxx0tz.pdf (3.02 MB)

Supplementary Figures and Tables from Functional Characterization of Brain Tumor-Initiating Cells and Establishment of GBM Preclinical Models that Incorporate Heterogeneity, Therapy, and Sex Differences

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journal contribution
posted on 2023-04-03, 18:48 authored by Cesar A. Garcia, Adip G. Bhargav, Mieu Brooks, Paola Suárez-Meade, Sujan K. Mondal, Natanael Zarco, Karim ReFaey, Mark Jentoft, Erik H. Middlebrooks, Matija Snuderl, Anna Carrano, Hugo Guerrero-Cazares, Paula Schiapparelli, Rachel Sarabia-Estrada, Alfredo Quiñones-Hinojosa

Supplementary Figures and Tables


Mayo Clinic Professorship, a Clinician Investigator award

the Florida Department of Health Cancer Research Chair Fund


NCI Diversity Supplement Program



Glioblastoma (GBM) is the most common primary brain cancer in adults where tumor cell heterogeneity and sex differences influence clinical outcomes. Here, we functionally characterize three male and three female patient-derived GBM cell lines, identify protumorigenic BTICs, and create novel male and female preclinical models of GBM. Cell lines were evaluated on the following features: proliferation, stemness, migration, tumorigenesis, clinical characteristics, and sensitivity to radiation, TMZ, rhTNFSF10 (rhTRAIL), and rhBMP4. All cell lines were classified as GBM according to epigenetic subtyping, were heterogenous and functionally distinct from one another, and re-capitulated features of the original patient tumor. In establishing male and female preclinical models, it was found that two male-derived GBM cell lines (QNS108 and QNS120) and one female-derived GBM cell line (QNS315) grew at a faster rate in female mice brains. One male-derived GBM cell line (QNS108) decreased survival in female mice in comparison with male mice. However, no survival differences were observed for mice injected with a female-derived cell line (QNS315). In summary, a panel of six GBM patient-derived cell lines were functionally characterized, and it was shown that BTIC lines can be used to construct sex-specific models with differential phenotypes for additional studies.