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Supplementary Figures and Tables S1 and S3 from Infiltration of Tumors Is Regulated by T cell–Intrinsic Nitric Oxide Synthesis

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posted on 2023-04-04, 02:02 authored by Pedro P. Cunha, David Bargiela, Eleanor Minogue, Lena C.M. Krause, Laura Barbieri, Carolin Brombach, Milos Gojkovic, Emilia Marklund, Sandra Pietsch, Iosifina Foskolou, Cristina M. Branco, Pedro Veliça, Randall S. Johnson

Supplementary Figures S1-S6, Supplementary Tables S1 and S3, and uncropped western blot images. 

Funding

Wellcome Trust (WT)

Knut och Alice Wallenbergs Stiftelse (Knut and Alice Wallenberg Foundation)

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ARTICLE ABSTRACT

Nitric oxide (NO) is a signaling molecule produced by NO synthases (NOS1–3) to control processes such as neurotransmission, vascular permeability, and immune function. Although myeloid cell–derived NO has been shown to suppress T-cell responses, the role of NO synthesis in T cells themselves is not well understood. Here, we showed that significant amounts of NO were synthesized in human and murine CD8+ T cells following activation. Tumor growth was significantly accelerated in a T cell–specific, Nos2-null mouse model. Genetic deletion of Nos2 expression in murine T cells altered effector differentiation, reduced tumor infiltration, and inhibited recall responses and adoptive cell transfer function. These data show that endogenous NO production plays a critical role in T cell–mediated tumor immunity.

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    Cancer Immunology Research

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