journal contribution
posted on 2023-04-03, 23:23 authored by Ji Cao, Rong Dong, Li Jiang, Yanling Gong, Meng Yuan, Jieqiong You, Wen Meng, Zhanlei Chen, Ning Zhang, Qinjie Weng, Hong Zhu, Qiaojun He, Meidan Ying, Bo Yang Supplementary figures and figure legends
Funding
National Natural Science Foundation of China
Department of Education of Zhejiang Province
Zhejiang Provincial Natural Science Foundation
Medical Science and Technology Planning Project of Zhejiang Province
Talent Project of Zhejiang Association for Science and Technology
History
ARTICLE ABSTRACT
M2 polarization of macrophages is essential for their function in immunologic tolerance, which might promote tumorigenesis. However, the molecular mechanism behind the polarization process is not fully understood. Given that several lines of evidence have suggested that long noncoding RNAs (lncRNAs) could be involved in regulating immune cell differentiation and function, the current study aimed to identify the lncRNAs that specifically modulate M2 macrophage polarization. By utilizing a series of cell-based M2 macrophage polarization models, a total of 25 lncRNAs with altered expression were documented based on lncRNA microarray-based profiling assays. Among them, lncRNA-MM2P was the only lncRNA upregulated during M2 polarization but downregulated in M1 macrophages. Knockdown of lncRNA-MM2P blocked cytokine-driven M2 polarization of macrophages and weakened the angiogenesis-promoting feature of M2 macrophages by reducing phosphorylation on STAT6. Moreover, manipulating lncRNA-MM2P in macrophages impaired macrophage-mediated promotion of tumorigenesis, tumor growth in vivo, and tumor angiogenesis. Collectively, our study identifies lncRNA-MM2P as a modulator required for macrophage M2 polarization and uncovers its role in macrophage-promoted tumorigenesis.
