American Association for Cancer Research
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Supplementary Figures 1 through 9 from Pseudogene PTENP1 Functions as a Competing Endogenous RNA to Suppress Clear-Cell Renal Cell Carcinoma Progression

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posted on 2023-04-03, 14:25 authored by Gan Yu, Weimin Yao, Kiranmai Gumireddy, Anping Li, Ji Wang, Wei Xiao, Ke Chen, Haibing Xiao, Heng Li, Kun Tang, Zhangqun Ye, Qihong Huang, Hua Xu

Supplementary Figure 1: PTENP1 and PTEN expression were decreased in human ccRCC samples. Supplementary Figure 2: PTENP1 expression in ACHN and SN12PM6 cells. Supplementary Figure 3: PTENP1 expression activated AKT in ACHN and SN12PM6 cells. Supplementary Figure 4: miR-21 expression in ACHN and SN12PM6 cells. Supplementary Figure 5: PTEN expression in ACHN and SN12PM6 cells. Supplementary Figure 6: 5-10 fold overexpression of PTENP1 increased PTEN expression and suppressed cell proliferation, migration and invasion. Supplementary Figure 7: PTENP1 functions and regulation of PTEN are DICER dependent. Supplementary Figure 8: 5'AZA increased PTENP1 and PTEN expression and suppressed cell proliferation, migration and invasion. Supplementary Figure 9: Cisplatin and gemcitabine treatment increased PTENP1 expression.

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ARTICLE ABSTRACT

PTENP1 is a pseudogene of the PTEN tumor suppression gene (TSG). The functions of PTENP1 in clear-cell renal cell carcinoma (ccRCC) have not yet been studied. We found that PTENP1 is downregulated in ccRCC tissues and cells due to methylation. PTENP1 and PTEN are direct targets of miRNA miR21 and their expression is suppressed by miR21 in ccRCC cell lines. miR21 expression promotes ccRCC cell proliferation, migration, invasion in vitro, and tumor growth and metastasis in vivo. Overexpression of PTENP1 in cells expressing miR21 reduces cell proliferation, invasion, tumor growth, and metastasis, recapitulating the phenotypes induced by PTEN expression. Overexpression of PTENP1 in ccRCC cells sensitizes these cells to cisplatin and gemcitabine treatments in vitro and in vivo. In clinical samples, the expression of PTENP1 and PTEN is correlated, and both expressions are inversely correlated with miR21 expression. Patients with ccRCC with no PTENP1 expression have a lower survival rate. These results suggest that PTENP1 functions as a competing endogenous RNA (ceRNA) in ccRCC to suppress cancer progression. Mol Cancer Ther; 13(12); 3086–97. ©2014 AACR.