Supplementary Figures 1 through 11 from Phospholipase D1 Acts through Akt/TopBP1 and RB1 to Regulate the E2F1-Dependent Apoptotic Program in Cancer Cells

<p>Supplementary Figure S1. PLD1 inactivation promotes apoptosis under serum deprived condition. Supplementary Figure S2. Scatter diagrams of gene expression microarray by depletion of PLD1. Supplementary Figure S3. PLD1 inactivation decreases RB1 expression in the tumor tissues from ApcMin/+ mice. Supplementary Figure S4. Loss of PLD1 decreases RB1 expression in the tumor tissues from AOM/DSS mice. Supplementary Figure S5. Epigenetic regulation of RB1 promoter is not affected by depletion and inhibition of PLD1. Supplementary Figure S6. Pre-miR-4465 and -192 decrease RB1 mRNA level. Supplementary Figure S7. IEC-specific PLD1 overexpression in ApcMin/+ mice increases the levels of RB1 and p-Akt. Supplementary Figure S8. PLD1 inhibition decreases Akt phosphorylation in AOM/DSS mice. Supplementary Figure S9. Effect of depletion of p27KIP1, Bim, or NOXA on E2F1-induced apoptosis. Supplementary Figure S10. Effect of RB1, Akt, and anti-miR-4496/-192 on PLD1 inhibition-repressed beta-catenin and c-Myc expression. Supplementary Figure S11. Multivariate survival analysis between expression of PLD1 and proapoptotic E2F1 target genes in CRC patients.</p>