Supplementary Figures 1 - 8 from miRNA Biomarkers in Cyst Fluid Augment the Diagnosis and Management of Pancreatic Cysts
PDF file, 917K, Supplemental Figure 1: (A) Laser microdissection in an IPMN (hematoxylin and eosin; original magnification: 10x); (B) After precise marking and microdissection the neoplastic epithelium is catapulted into a sterile tube for subsequent RNA extraction. (C) The section after dissection. Supplemental Figure 2: Detailed diagram of the study design, describing experimental setup of FFPE (FTS) and cyst fluid (CFS) studies, including number of specimens used and number of miRNA candidates identified in the course of each study. Supplemental Figure 3: Mean Cts for all FTS and CFS specimens. (A) Megaplex RT-qPCR data for FTS. (B) and (C) Singleplex RT-qPCR data for FTS. (D) Megaplex RT-qPCR data for CFS. (E) and (F) Singleplex RT-qPCR data for CFS. Supplemental Figure 4: Boxplots showing raw Ct values for Megaplex (A) and singleplex (B) RT-qPCR expression analyses of FTS1 and FTS1 plus FTS2, respectively. The diagnosis associated with each sample is indicated by color, while the FTS1 and FTS2 specimens are depicted as separate panels in the singleplex boxplot (B). Supplemental Figure 5: Boxplots showing raw Ct values for Megaplex (A) and singleplex (B) RT-qPCR expression analyses of cyst fluid specimens from the CFS1 and CFS1 plus CFS2, respectively. Diagnosis is indicated by color, the CFS1 and CFS2 specimens by panel. Supplemental Figure 6: Boxplots of raw Ct values for singleplex RT-qPCR expression analysis of the CFS1 and CFS2 specimens with reassignment to test or training set indicated by separation of panels. Supplemental Figure 7: Raw Ct values of miRNAs involved in DiffPairs comprising the logistic regression model in the CFS1 and CFS2 specimens. Raw Ct for miR-21, which is not a part of the logistic regression model, are also shown. Supplemental Figure 8: PCA applied to raw Cts (A) and restricted mean-center normalized Cts. (B) CFS1 and CFS2 singleplex RT-qPCR data. Note that CFS1 and CFS2 specimens do not separate in either plot.