American Association for Cancer Research
00085472can132689-sup-can-13-2689fig1-7tab1-3.pdf (1.7 MB)

Supplementary Figures 1 - 7, Tables 1 - 3 from Fatty Acid-Binding Protein E-FABP Restricts Tumor Growth by Promoting IFN-β Responses in Tumor-Associated Macrophages

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journal contribution
posted on 2023-03-30, 22:30 authored by Yuwen Zhang, Yanwen Sun, Enyu Rao, Fei Yan, Qiang Li, Ying Zhang, Kevin A.T. Silverstein, Shujun Liu, Edward Sauter, Margot P. Cleary, Bing Li

PDF file - 1745K, Supplementary Figure 1. The development of tumors in WT and E-FABP-/- mice. Supplementary Figure 2. Phenotype analysis of tumor-bearing WT and E-FABP-/- mice. Supplementary Figure 3. Dynamic changes of E-FABP-expressing TAMs in the tumor stroma. Supplementary Figure 4. Analysis of GM-BMMs from WT and E-FABP-/- mice. Supplementary Figure 5. Analysis of FABP expression and LD inhibition in GM-BMMs. Supplementary Figure 6. IFNγ production in tumor infiltrated cells and NK depletion in mice. Supplementary Figure 7. E-FABP expression in breast cancer patients. Supplementary Table 1. The selected differentially expressed genes in macrophages regulated by E-FABP. Supplementary Table 2. E-FABP expression in normal breast tissues and breast cancer tissues. Supplementary Table 3. E-FABP expression in normal breast stroma and invasive breast cancer stroma.



Fatty acid-binding proteins (FABP) are known central regulators of both metabolic and inflammatory pathways, but their role in tumor development remains largely unexplored. Here, we report that host expression of epidermal FABP (E-FABP) protects against mammary tumor growth. We find that E-FABP is highly expressed in macrophages, particularly in a specific subset, promoting their antitumor activity. In the tumor stroma, E-FABP–expressing tumor-associated macrophages (TAM) produce high levels of IFN-β through upregulation of lipid droplet formation in response to tumors. E-FABP–mediated IFN-β signaling can further enhance recruitment of tumoricidal effector cells, in particular natural killer cells, to the tumor stroma for antitumor activity. These findings identify E-FABP as a new protective factor to strengthen IFN-β responses against tumor growth. Cancer Res; 74(11); 2986–98. ©2014 AACR.

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