Supplementary Figures 1-9 from VEGF Exerts an Angiogenesis-Independent Function in Cancer Cells to Promote Their Malignant Progression

PDF file - 824K, 1) Supplementary Figure S1. VEGF knock down in RCC cells restricted the tumor size and induced a differentiation phenotype in vivo. 2) Supplementary Figure S2. VEGF knock down in RCC cells restricted the tumor phenotypes in vitro. 3) Supplementary Figure S3. VEGF knockdown did not affect 786-O and A-498 cell proliferation and apoptosis. 4) Supplementary Figure S4. Induction of VEGF shRNA ((+)Dox) reduced A-498 cells anchorage independent clonogenic ability. 5) Supplementary Figure S5. Low concentration (0.2ug/ml) Doxycycline did not have a significant effect on A-498 cell proliferation, apoptosis, differentiation, or tumor formation. 6) Supplementary Figure S6. Mouse xenograft model of VEGF in tumorigenesis using a GFP/RFP combination system. 7) Supplementary Figure S7. The distribution of GFP and RFP labeled cells and the vascular densities in the tumor tissue. 8) Supplementary Figure S8. ERK1/2 and AKT were activated by VEGF. 9) Supplementary Figure S9. Knockdown of SOS1/2 induced 786-O cells into a differentiation phenotype.