American Association for Cancer Research
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Supplementary Figure SF1 from Novel Paired Normal Prostate and Prostate Cancer Model Cell Systems Derived from African American Patients

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posted on 2023-04-04, 02:04 authored by Mira Jung, Keith Kowalczyk, Ryan Hankins, Gaurav Bandi, Bhaskar Kallakury, Michael A. Carrasquilla, Partha P. Banerjee, Scott Grindrod, Anatoly Dritschilo

Figure S1: A) Results obtained from analyses of 15 autosomal short tandem repeat (STR) loci identify the male locus amelogenin. The profile of L/P4T is unique. No mouse DNA was detected for this sample. B) Karyotype of AP4T. Of 20 metaphases counted, 5 metaphases were analyzed. 3 metaphases were then karyotyped at 425 banding resolution. The image shows the 46,XY, male karyotype. Normal karyotype (male) within the limits of resolution attained. C) Cell growth and morphology under CR condition. Non-cancerous and cancerous cells derived from AP4 were expanded using CR condition. The images of 10x and 20x are from passage 1 and stained with H&E. The bottom image shows clonogenic growth of epithelial cells and J2 fibroblasts after 4 days and 7 days of culture. On day 7, clonogenic epithelial cells are marked in yellow arrow and J2 fibroblasts in cyan blue arrow.

Funding

HHS | NIH | NCI | Cancer Moonshot (Misión contra el Cáncer)

HHS | NIH | National Cancer Institute (NCI)

History

ARTICLE ABSTRACT

Cells derived from prostatectomies of AA patients conferred a bimodal cellular phenotype, recapitulating clinical prostate cellular complexity in this model cell system. Comparisons of viability responses of tumor derived to normal epithelial cells offer the potential for screening therapeutic drugs. Therefore, these paired prostate epithelial cell cultures provide an in vitro model system suitable for studies of molecular mechanisms in health disparities.