Supplementary Figure S5 from Small Molecule–Mediated Activation of RAS Elicits Biphasic Modulation of Phospho-ERK Levels that Are Regulated through Negative Feedback on SOS1
posted on 2023-04-03, 15:07authored byJennifer E. Howes, Denis T. Akan, Michael C. Burns, Olivia W. Rossanese, Alex G. Waterson, Stephen W. Fesik
SPRY2 is not phosphorylated on tyrosine residues in response to 1 treatment. 293 cells were transiently transfected with V5-SPRY2 and treated with 25 μM 1 over a timecourse of up to 90 minutes. Where indicated, cells were treated with FBS (20% v/v) for 15 minutes. Two biological repeats of this experiment were independently conducted.