American Association for Cancer Research
10780432ccr153028-sup-159506_1_supp_3366669_k2sdyk.docx (27.59 kB)

Supplementary Figure S2 from Genomic EWSR1 Fusion Sequence as Highly Sensitive and Dynamic Plasma Tumor Marker in Ewing Sarcoma

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journal contribution
posted on 2023-03-31, 19:45 authored by Manuela Krumbholz, Julia Hellberg, Benedikt Steif, Tobias Bäuerle, Clarissa Gillmann, Torsten Fritscher, Abbas Agaimy, Benjamin Frey, Joerg Juengert, Eva Wardelmann, Wolfgang Hartmann, Heribert Juergens, Uta Dirksen, Markus Metzler

Individual genomic EWSR1-FLI1 or EWSR1-ERG fusion sequences. Repeat regions are highlighted in gray.



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Purpose: The application of the tumor-specific genomic fusion sequence as noninvasive biomarker for therapy monitoring in Ewing sarcoma (EwS) has been evaluated.Experimental Design: EwS xenograft mouse models were used to explore detectability in small plasma volumes and correlation of genomic EWSR1-FLI1 copy numbers with tumor burden. Furthermore, 234 blood samples from 20 EwS patients were analyzed before and during multimodal treatment. EWSR1 fusion sequence levels in patients' plasma were quantified using droplet digital PCR and compared with tumor volumes calculated from MRI or CT imaging studies.Results: Kinetics of EWSR1 fusion sequence copy numbers in the plasma are correlated with changes of the tumor volume in patients with localized and metastatic disease. The majority of patients showed a fast reduction of cell-free tumor DNA (ctDNA) during initial chemotherapy. Recurrence of increasing ctDNA levels signalized relapse development.Conclusions: Genomic fusion sequences represent promising noninvasive biomarkers for improved therapy monitoring in EwS. Until now, response assessment is largely based on MRI and CT imaging, implying restrictions on closely repeated performance and limitations on the differentiation between vital tumor and reactive stromal tissue. Particularly in patients with prognostic unfavorable disseminated disease, ctDNA is a valuable addition for the assessment of therapy response. Clin Cancer Res; 22(17); 4356–65. ©2016 AACR.

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