American Association for Cancer Research
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Supplementary Figure S1 from Neutrophils Mediate Protection Against Colitis and Carcinogenesis by Controlling Bacterial Invasion and IL22 Production by γδ T Cells

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posted on 2024-04-02, 07:23 authored by Silvia Carnevale, Andrea Ponzetta, Anna Rigatelli, Roberta Carriero, Simone Puccio, Domenico Supino, Giovanna Grieco, Piera Molisso, Irene Di Ceglie, Francesco Scavello, Chiara Perucchini, Fabio Pasqualini, Camilla Recordati, Claudio Tripodo, Beatrice Belmonte, Andrea Mariancini, Paolo Kunderfranco, Giuseppe Sciumè, Enrico Lugli, Eduardo Bonavita, Elena Magrini, Cecilia Garlanda, Alberto Mantovani, Sebastien Jaillon

Figure S1. Neutrophil deficiency is associated with increased lymphoid aggregates. Related to Figure 1.A) Number of total lymphoid structures and B) number of lymphoid structures with follicular dendritic cells detected by RNAscope staining for the follicular dendritic cell marker Mfge8 in colon tissue sections from Csf3r+/+(n=8) and Csf3r-/- (n=5) mice after AOM/DSS-induced CRC. C) Representative images of RNAscope analysis for Mfge8 in colon tissue sections from Csf3r-/- mice after AOM/DSS-induced CRC: aggregates with follicular structures are visible as Mfge8+ (red spot): on the top is visible a lymphoid aggregate at two different magnifications, with diffuse Mfge8+ cells; on the bottom right lymphoid microaggregate with Mfge8+ cells; on the bottom left aggregate without follicular structures (Mfge8+). D) Representative dot-plot of neutrophils frequency in blood (top panels) and colon LP (bottom panels) of Csf3r-/- mice and Csf3r-/- mice 4hrs and 18hrs after adoptive transfer of neutrophils. E-F) Frequency of neutrophils in blood (D) and colon LP (E) of DSS-treated Csf3r-/- mice (n=5) and Csf3r-/- mice 4hrs (n=5) and 18hrs (n=5) after adoptive transfer of neutrophils. A-B) Representative data of three independent experiments. C-E) One experiment. A-B, D-E) Unpaired Student’s t-Test. Data are mean ± SEM. *** p < 0.001 ** p < 0.01 * p < 0.05.


Ministero della Salute (Italy Ministry of Health)

Fondazione AIRC per la ricerca sul cancro ETS (AIRC)

Ministero dell'Università e della Ricerca (MUR)



Neutrophils are the most abundant leukocytes in human blood and play a primary role in resistance against invading microorganisms and in the acute inflammatory response. However, their role in colitis and colitis-associated colorectal cancer is still under debate. This study aims to dissect the role of neutrophils in these pathologic contexts by using a rigorous genetic approach. Neutrophil-deficient mice (Csf3r−/− mice) were used in classic models of colitis and colitis-associated colorectal cancer and the role of neutrophils was assessed by histologic, cellular, and molecular analyses coupled with adoptive cell transfer. We also performed correlative analyses using human datasets. Csf3r−/− mice showed increased susceptibility to colitis and colitis-associated colorectal cancer compared with control Csf3r+/+ mice and adoptive transfer of neutrophils in Csf3r−/− mice reverted the phenotype. In colitis, Csf3r−/− mice showed increased bacterial invasion and a reduced number of healing ulcers in the colon, indicating a compromised regenerative capacity of epithelial cells. Neutrophils were essential for γδ T-cell polarization and IL22 production. In patients with ulcerative colitis, expression of CSF3R was positively correlated with IL22 and IL23 expression. Moreover, gene signatures associated with epithelial-cell development, proliferation, and antimicrobial response were enriched in CSF3Rhigh patients. Our data support a model where neutrophils mediate protection against intestinal inflammation and colitis-associated colorectal cancer by controlling the intestinal microbiota and driving the activation of an IL22-dependent tissue repair pathway.

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