Supplementary Figure S16 from Single-cell Transcriptional Atlas of Human Hematopoiesis Reveals Genetic and Hierarchy-Based Determinants of Aberrant AML Differentiation

<p>Supplementary Figure S16. Sorting strategy for primary and xenografted AML samples A) Gating strategy for primary AML samples sorted into immature CD34+CD38- and mature CD34-CD38+ fractions, pertaining to Figure 7 and Supplementary Figures S17, S18, and S19. Live (PI-) single cells were gated on CD45+CD3- prior to sorting based on CD34 and CD38 status. Sorted immature CD34+CD38- and mature CD34-CD38+ fractions were subject to xenotransplantation as well as scRNA-seq. B) Gating strategy for AML xenograft samples profiled for scRNA-seq, pertaining to Figure 7 and Supplementary Figures S17, S18, and S19. Live (PI-) single cells were gated on CD45+CD3- prior to scRNA-seq. Detailed information on antibodies used for fluorescence-activated cell sorting (FACS) can be found in Supplementary Table S21.</p>