American Association for Cancer Research
Browse

Supplementary Figure S1-S5 from Long Noncoding RNA pancEts-1 Promotes Neuroblastoma Progression through hnRNPK-Mediated β-Catenin Stabilization

Download (616.36 kB)
journal contribution
posted on 2023-03-31, 00:12 authored by Dan Li, Xiaojing Wang, Hong Mei, Erhu Fang, Lin Ye, Huajie Song, Feng Yang, Huanhuan Li, Kai Huang, Liduan Zheng, Qiangsong Tong

Figure S1 shows the schematic positions for primers; Figure S2 shows the characterization of a novel lncRNA pancEts-1; Figure S3 shows the expression and Kaplan-Meier survival curves of pancEts-1 downstream genes; Figure S4 shows the expression of β-catenin and hnRNPK in NB cells; Figure S5 shows the roles of pancEts-1 in facilitating the β-catenin activity and downstream gene expression in NB cells.

Funding

National Natural Science Foundation of China

Fundamental Research Funds for the Central Universities

Natural Science Foundation of Hubei Province

History

ARTICLE ABSTRACT

Long noncoding RNAs (lncRNA) play essential roles in tumor progression. However, the functions of lncRNAs in the tumorigenesis and aggressiveness of neuroblastoma still remain to be determined. Here, we report the identification of lncRNA pancEts-1 as a novel driver of neuroblastoma progression by using a public microarray dataset. LncRNA pancEts-1 promoted the growth, invasion, and metastasis of neuroblastoma cells in vitro and in vivo. Mechanistically, pancEts-1 bound to hnRNPK to facilitate its physical interaction with β-catenin, whereas hnRNPK stabilized the β-catenin by inhibiting proteasome-mediated degradation, resulting in transcriptional alteration of target genes associated with neuroblastoma progression. Both pancEts-1 and hnRNPK were upregulated in clinical neuroblastoma tissues, and were associated with unfavorable outcome of patients. Overall, our results define an oncogenic role of pancEts-1 in neuroblastoma progression through hnRNPK-mediated β-catenin stabilization, with potential implications for the clinical therapeutics of neuroblastoma.Significance: These findings reveal the oncogenic functions of a long noncoding RNA in neuroblastoma progression, offering a potential target for clinical therapeutics. Cancer Res; 78(5); 1169–83. ©2018 AACR.

Usage metrics

    Cancer Research

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC