Supplementary Figure Legend from Estrogen Receptor α Regulates ATM Expression through miRNAs in Breast Cancer

<p>Supplementary Figure Legend - PDF file 60K, Supplementary Figure S1: top panel: MCF-7 cells were transiently transfected with control of ER-alpha siRNA. RNA was extracted 48 hours after transfected and the level of ATM mRNA was measured by RT-PCR. Bottom panel: Expression of ATM mRNA in MCF-7 cells by Real-time PCR. Expression levels were normalized by GAPDH. Error bars represent mean SD calculated from 3 parallel experiments. Supplemental Figure S2: Predicted consequential pairing of target region of ATM (top) and miRNA (bottom) of MiRNAs 18a, 106a and 101. Supplemental Figure S3: MDA-MB-231 cells were co-transfected with a luciferase reporter plasmid along with miRNA-18a or miRNA 106a. Relative luciferase activities representing ATM 3'UTR and pMirTarget vector are presented. Shown are averages of triplicate samples and standard deviations. Student t tests were used to analyze the data. Supplemental Figure S4: MiRNAs 18a and 106a are under-expressed in ER negative breast cancer tissues. In situ hybridization was conducted in 296 cases of breast cancer tissues probing miRNAs 18a and 106a. Shown are representative images of miRNA 18a and 106 expressions</p>