American Association for Cancer Research
00085472can131299-sup-figure_8.pdf (1.7 MB)

Supplementary Figure 8 from Comparative Expression Analysis Reveals Lineage Relationships between Human and Murine Gliomas and a Dominance of Glial Signatures during Tumor Propagation In Vitro

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journal contribution
posted on 2023-03-30, 21:55 authored by Nico V. Henriquez, Tim Forshew, Ruth Tatevossian, Matthew Ellis, Angela Richard-Loendt, Hazel Rogers, Thomas S. Jacques, Pablo Garcia Reitboeck, Kerra Pearce, Denise Sheer, Richard G. Grundy, Sebastian Brandner

PDF file, 1743K, p53 sequencing of Rb/Pten tumours and in vitro recombined Rb/Pten neurospheres.



Brain tumors are thought to originate from stem/progenitor cell populations that acquire specific genetic mutations. Although current preclinical models have relevance to human pathogenesis, most do not recapitulate the histogenesis of the human disease. Recently, a large series of human gliomas and medulloblastomas were analyzed for genetic signatures of prognosis and therapeutic response. Using a mouse model system that generates three distinct types of intrinsic brain tumors, we correlated RNA and protein expression levels with human brain tumors. A combination of genetic mutations and cellular environment during tumor propagation defined the incidence and phenotype of intrinsic murine tumors. Importantly, in vitro passage of cancer stem cells uniformly promoted a glial expression profile in culture and in brain tumors. Gene expression profiling revealed that experimental gliomas corresponded to distinct subclasses of human glioblastoma, whereas experimental supratentorial primitive neuroectodermal tumors (sPNET) correspond to atypical teratoid/rhabdoid tumor (AT/RT), a rare childhood tumor. Cancer Res; 73(18); 5834–44. ©2013 AACR.