American Association for Cancer Research
Browse
00085472can076346-sup-s_fig_7.pdf (1.67 MB)

Supplementary Figure 7 from A Role for the Cyclin Box in the Ubiquitin-Mediated Degradation of Cyclin G1

Download (1.67 MB)
journal contribution
posted on 2023-03-30, 18:07 authored by Denise M. Piscopo, Philip W. Hinds
Supplementary Figure 7 from A Role for the Cyclin Box in the Ubiquitin-Mediated Degradation of Cyclin G1

History

ARTICLE ABSTRACT

Cyclin G1 was identified as a transcriptional target of p53 that encodes a protein with strong homology to the cyclin family of cell cycle regulators. We show that either ectopically expressed or endogenous cyclin G1 protein is very unstable, undergoes modification with ubiquitin, and is likely degraded by the proteasome. Ectopic cyclin G1 protein stability is increased by cyclin box mutation or by association with inactive cyclin-dependent kinase (CDK) subunits, suggesting that a function of cyclin G1 as a CDK regulator may be required for its rapid turnover. Furthermore, cyclin G1 and the cyclin box mutant interact with and are ubiquitinated by MDM2, another transcriptional target of p53 that acts as a negative regulator of p53 stability. These data suggest that the cyclin box has a role in the proteasome-mediated degradation of cyclin G1 and thus suggest a putative role for a CDK in cyclin G1 metabolism and function. [Cancer Res 2008;68(14):5581–90]

Usage metrics

    Cancer Research

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC