posted on 2023-03-30, 22:24authored byJ. Louise Lines, Eirini Pantazi, Justin Mak, Lorenzo F. Sempere, Li Wang, Samuel O'Connell, Sabrina Ceeraz, Arief A. Suriawinata, Shaofeng Yan, Marc S. Ernstoff, Randolph Noelle
PDF file - 77KB, VISTA does not affect B cell proliferation. CD19 positive B cells were purified from human PBMCs by magnetic bead negative selection. (a and b) cells were stimulated in the presence of VISTA-Ig (a) or control Ig (b) at 5ug/ml for 4 days with 250ng/ml of CD40 agonist. Plots show representative CFSE profiles gated on live CD19 positive events. C) percent proliferation in the presence of decreasing concentrations of agonist in the presence of VISTA-Ig (filled bars) or control Ig (open bars) at 5ug/ml for 4 days. Data is representative of 3 separate experiments.