Supplementary Figure 5 from Loss of miR-204 Expression Enhances Glioma Migration and Stem Cell-like Phenotype
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posted on 2023-03-30, 21:52 authored by Zhe Ying, Yun Li, Jueheng Wu, Xun Zhu, Yi Yang, Han Tian, Wei Li, Bo Hu, Shi-Yuan Cheng, Mengfeng LiPDF file - 22K, The effect of de-metylation agent on the expression of SOX4 and EphB2 in glioma cells.
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ARTICLE ABSTRACT
Phenotypic similarities have long been recognized between subpopulations of glioma and neural stem cells. Many of these similar properties, including the robust abilities to self-renew, migrate, and invade, are hallmarks of glioma cells that render them extremely aggressive. However, the molecular mechanisms underlying this character, particularly in glioma stem-like cells that drive this disease, remain poorly understood. Here, we report the results of a differential miRNA expression screen that compared glioma and neural stem cells, where we found that miR-204 was markedly downregulated in both types of cells. Mechanistic investigations revealed that miR-204 simultaneously suppressed self-renewal, stem cell-associated phenotype, and migration of glioma cells by targeting the stemness-governing transcriptional factor SOX4 and the migration-promoting receptor EphB2. Restoring miR-204 expression in glioma cells suppressed tumorigenesis and invasiveness in vivo and increased overall host survival. Further evaluation revealed that the miR-204 promoter was hypermethylated and that attenuating promoter methylation was sufficient to upregulate miR-204 in glioma cells. Together, our findings reveal miR-204 as a pivotal regulator of the development of stem cell-like phenotypes and cell motility in malignant glioma cells. Cancer Res; 73(2); 990–9. ©2012 AACR.Usage metrics
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