posted on 2023-03-30, 20:46authored byAlessia Lopergolo, Michele Tavecchio, Sofia Lisanti, Jagadish C. Ghosh, Takehiko Dohi, Alice Faversani, Valentina Vaira, Silvano Bosari, Nobuhiko Tanigawa, Domenico Delia, Andrew V. Kossenkov, Louise C. Showe, Dario C. Altieri
PDF file - 93K, Multiparametric flow cytometry detection of GammaH2AX reactivity. HCT116 cells were transfected with vector, WT survivin (SVV), survivin-DeltaEx3 or Chk2 phosphorylation-defective survivin-DeltaEx3-Mut3 cDNA, treated with etoposide (A) or camptothecin (B), and analyzed after 16 h for GammaH2AX (y-axis) and PI (x-axis) staining by multiparametric flow cytometry. In panel A, numbers in parenthesis correspond to GammaH2AX fluorescence units after background subtraction.