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Supplementary Figure 3, page 2 of 4 from Multiple Alternative Splicing Markers for Ovarian Cancer

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posted on 2023-03-30, 18:03 authored by Roscoe Klinck, Anne Bramard, Lyna Inkel, Geneviève Dufresne-Martin, Julien Gervais-Bird, Richard Madden, Éric R. Paquet, ChuShin Koh, Julian P. Venables, Panagiotis Prinos, Manuela Jilaveanu-Pelmus, Raymund Wellinger, Claudine Rancourt, Benoit Chabot, Sherif Abou Elela
Supplementary Figure 3, page 2 of 4 from Multiple Alternative Splicing Markers for Ovarian Cancer

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ARTICLE ABSTRACT

Intense efforts are currently being directed toward profiling gene expression in the hope of developing better cancer markers and identifying potential drug targets. Here, we present a sensitive new approach for the identification of cancer signatures based on direct high-throughput reverse transcription-PCR validation of alternative splicing events. This layered and integrated system for splicing annotation (LISA) fills a gap between high-throughput microarray studies and high-sensitivity individual gene investigations, and was created to monitor the splicing of 600 cancer-associated genes in 25 normal and 21 serous ovarian cancer tissues. Out of >4,700 alternative splicing events screened, the LISA identified 48 events that were significantly associated with serous ovarian tumor tissues. In a further screen directed at 39 ovarian tissues containing cancer pathologies of various origins, our ovarian cancer splicing signature successfully distinguished all normal tissues from cancer. High-volume identification of cancer-associated splice forms by the LISA paves the way for the use of alternative splicing profiling to diagnose subtypes of cancer. [Cancer Res 2008;68(3):657–63]

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