Supplementary Figure 3 from Targeting Plasminogen Activator Inhibitor-1 Inhibits Angiogenesis and Tumor Growth in a Human Cancer Xenograft Model

<p>PDF - 1214KB, A, About 103 HeLa were seeded in 6 well plates exposed to tiplaxtinin at the indicated concentration for 72 hrs followed by aspiration of culture media and replacement with fresh complete media. HeLa clones (HeLa-PAI-1OE-12, HeLa-PAI-1OE-12 and HeLaEmpty control) overexpressing PAI-1 were also plated similarly. Cells were incubated for an additional 14 days. After 14 days, colonies were fixed with 6.0% glutaraldehyde, stained with 0.5% crystal violet and counted under light microscopy. Data were represented as mean + SD of three independent replicates. B, About 2 x 103 HeLa cells pretreated with tiplaxtinin at the indicated concentration and HeLa clones (HeLa-PAI-1OE-12, HeLa-PAI-1OE-12 and HeLaEmpty control) were mixed in agar solution and fresh complete media and seeded in 96-well plates. Cells were incubated for 6-8 days. Colony formation was quantified using the fluorescent cell stain CyQuant GR Dye (Cell Biolabs Inc., San Diego, CA) in the FLUOstar Optima Reader. Data were represented as mean + SD of three independent replicates. All experiments were repeated at least three times. Targeting PAI-1 was noted to retard cellular proliferation and colony formation. *, p < 0.05</p>