American Association for Cancer Research
00085472can132591-sup-fig3.pdf (101.39 kB)

Supplementary Figure 3 from Slug Promotes Survival during Metastasis through Suppression of Puma-Mediated Apoptosis

Download (101.39 kB)
journal contribution
posted on 2023-03-30, 22:34 authored by Seaho Kim, Jiahong Yao, Kimita Suyama, Xia Qian, Bin-Zhi Qian, Sanmay Bandyopadhyay, Olivier Loudig, Carlos De Leon-Rodriguez, Zhen Ni Zhou, Jeffrey Segall, Fernando Macian, Larry Norton, Rachel B. Hazan

PDF file - 97KB, Supplementary Fig. S3. FGFR controls Slug expression independently of ERK or AKT activation.



Tumor cells must overcome apoptosis to survive throughout metastatic dissemination and distal organ colonization. Here, we show in the Polyoma Middle T mammary tumor model that N-cadherin (Cdh2) expression causes Slug (Snai2) upregulation, which in turn promotes carcinoma cell survival. Slug was dramatically upregulated in metastases relative to primary tumors. Consistent with a role in metastasis, Slug knockdown in carcinoma cells suppressed lung colonization by decreasing cell survival at metastatic sites, but had no effect on tumor cell invasion or extravasation. In support of this idea, Slug inhibition by shRNA sensitized tumor cells to apoptosis by DNA damage, resulting in caspase-3 and PARP cleavage. The prosurvival effect of Slug was found to be caused by direct repression of the proapoptotic gene, Puma (Bbc3), by Slug. Consistent with a pivotal role for a Slug–Puma axis in metastasis, inhibition of Puma by RNA interference in Slug-knockdown cells rescued lung colonization, whereas Puma overexpression in control tumor cells suppressed lung metastasis. The survival function of the Slug–Puma axis was confirmed in human breast cancer cells, where Slug knockdown increased Puma expression and inhibited lung colonization. This study demonstrates a pivotal role for Slug in carcinoma cell survival, implying that disruption of the Slug–Puma axis may impinge on the survival of metastatic cells. Cancer Res; 74(14); 3695–706. ©2014 AACR.

Usage metrics

    Cancer Research



    Ref. manager